Eşek sütü ve bazı bileşenlerinin in vitro ortamda antikanser/yara iyileştirme aktivitesinin incelenmesi

Erdal Eroğlu, Cemre Öksüz

Öz


Bu çalışmada, eşek sütü, kazein ve kesik süt suyu proteininin in vitro ortamda antikanser ve yara iyileştirme potansiyelinin araştırılması amaçlanmıştır. Öncelikle eşek sütünün pH değeri değiştirilerek kazein ve kesik süt suyu proteinleri elde edilmiştir. Eşek sütü, kazein ve kesik süt suyu proteininin in vitro ortamda insan prostat kanseri hücre hattı (PC-3) üzerindeki antikanser aktivitesi MTT hücre proliferasyon testi kullanılarak incelenmiş olup antikanser aktivite bulunamamıştır. MTT denemelerinde özellikle kazein proteininin %140 hücre proliferasyonuna sebep olduğu gözlenmiştir. Bu doğrultuda, insan fibroblast hücre hattı (BJ) üzerinde çizik testi uygulanarak bu proteinlerin hücre migrasyonu ve yara iyileştirici etkileri incelenmiştir. Eşek sütü, kazein ve kesik süt suyu proteini (3200 µg/ml) kontrol grubuna kıyasla hücre migrasyonunda ve in vitro yara kapanma hızında anlamlı artışa sebep olmuştur. Bu çalışma ile eşek sütünün ve içerisindeki diğer proteinlerin in vitro ortamda antikanser aktivite göstermeyip hücre proliferasyonuna sebep olduğu ve yara iyileştirme amaçlı kullanılabileceği gösterilmiştir.

Tam Metin:

PDF

Referanslar


https://www.cancer.gov/about-cancer/understanding/what-is-cancer, (03.12.2019).

Twilley, D. ve Lall, N., Chapter 7 - The Role of Natural Products From Plants in the Development of Anticancer Agents in Mandal, S. C., Mandal, V. ve Konishi, T., Natural Products and Drug Discovery: An Integrated Approach, Elsevier, 139-178, (2018).

https://hsgm.saglik.gov.tr/depo/birimler/kanser-db/istatistik/Turkiye_Kanser_Istatistikleri_2015.pdf, (03.12.2019)

Kaur, K., Kumar, V., Sharma, A. K. ve Gupta, G. K., Isoxazoline containing natural products as anticancer agents: A review, European Journal of Medicinal Chemistry, 77, 121-133, (2014).

Sah, B. N. P., Vasiljevic, T., McKechnie, S. ve Donkor, O. N., Identification of Anticancer Peptides from Bovine Milk Proteins and Their Potential Roles in Management of Cancer: A Critical Review, Comprehensive Reviews in Food Science and Food Safety, 14, 123-138, (2015).

Khazir, J., Riley, D. L., Pilcher, L. A., De-Maayer, P. D. ve Mir, B. A., Anticancer Agents from Diverse Natural Sources, 9, 11, 1655-1669, (2014).

Kurtoğlu, H. ve Karataş, A., Yara Tedavisinde Güncel Yaklaşımlar: Modern Yara Örtüleri, Ankara Eczacılık Fakültesi Dergisi, 38, 3, 211-232, (2009).

Yazar, H. ve Karaca, İ., Yumuşak Dokuda Yara İyileşmesi, Etkileyen Faktörler ve Skar Revizyonu, Atatürk Üniv. Diş. Hek. Fak. Dergisi,15, 152-161, (2016).

Guo, S., ve DiPietro, L. A., Factors Affecting Wound Healing, Journal of Dental Research, 89, 3, 219-229, (2010).

Pucar, L. B., Kovac, A., Detel, D., Buljevic, S., Pugel, P. E. ve Varljen, J., Wound Healing Process, Diabetes and Implications of Dipeptidyl Peptidase IV (DPPIV/CD26), Journal of Tissue Science & Engineering, 8, 3, (2017).

Pradhan, L., Andersen, N. D., Nabzdyk, C., LoGerfo, F. W. ve Veves, A., Wound-healing Abnormalities in Diabetes and New Therapeutic Interventions, US Endocrine Disease, 68-72, (2007).

Garraud, O., Hozzein, W. N. ve Badr, G., Wound healing: time to look for intelligent, ‘natural’ immunological approaches?, BMC Immunology, 18, 1, 23, (2017).

Krishnankutty, R., Iskandarani, A., Therachiyil, L., Uddin, S., Azizi, F., Kulinski, M., Bhat, A. A. ve Mohammad, R. M., Anticancer Activity of Camel Milk via Induction of Autophagic Death in Human Colorectal and Breast Cancer Cells, Asian Pacific Journal of Cancer Prevention, 19, 12, 3501-3509, (2018).

Budak, Ş. ve Gürsel, A., Alternatif Bir Süt: Eşek Sütü, GIDA, 37, 4, 243-250, (2012)

Li, L., Liu, X. ve Guo, H., The nutritional ingredients and antioxidant activity of donkey milk and donkey milk powder, Food Science and Biotechnology, 27, 393–400, (2018).

Polidori, P. ve Vincenzetti, S., Protein Profile Characterizaytion of Donkey Milk in Hurley, W., Milk Protein, In TechOpen, 215-232, (2012).

Devi, S., Gandhi, K., Rana, S., Arora, S. ve Kapila S., Donkey Milk: A Very Recent Nutritional Pharmafood, Indian Dairyman, 70, 72-77 (2018).

Taşçı, F., Eşek Stünün Özellikleri ve Gıda Alerjilerinde Kullanımı, Uludağ Üniversitesi Veterinerlik Fakültesi Dergisi, 30, 2, 39-44, (2011).

Sharitatikia, M., Behbahani, M. ve Mohabatkar, H., Anticancer activity of cow, sheep, goat, mare, donkey and camel milks and their caseins and whey proteins and in silico comparison of the caseins, Molecular Biology Research Communications, 6, 2, 57-64, (2017).

Dutta, S., Mahalanobish, S., Saha, S., Ghosh, S. ve Sil, P. C., Natural products: An upcoming therapeutic approach to cancer, Food and Chemical Toxicology, 128, 240–255, (2019).

Lalitha, L. J., Sales, T. J., Prince.P.Clarance, P.Agastian, Kim, Y. O., Mahmoud, A. H., Mohamed, S. E., Tack, J. C., Na, S. W. ve Kim, H. J., In-vitro phytopharmacological and anticancer activity of Loranthus Longiflorus Desv. Var. Falcatuskurz against the human lung cancer cells, Journal of King Saud University – Science, 32, 1246–1253, (2020).

Premarathna, A. D., Ranahewa, T. H., Wijesekera, S. K., Wijesundara, R. R. M. K. K., Jayasooriya, A. P., Wijewardana, V. ve Rajapakse, R. P. V. J., Wound healing properties of aqueous extracts of Sargassum illicifolium: An in vitro assay, Wound Medicine, 24, 1–7, (2019).

Mao, X., Gu, J., Sun, Y., Xu, S., Zhang, X., Yang, H. ve Ren, F., Anti-proliferative and anti-tumour effect of active components in donkey milk on A549 human lung cancer cells, International Dairy Journal 19, 11, 703-708, (2009).

Al-Mudallala, N. H., Tahab, A. A., Abbas, M. Q. ve Mohammedd, F. I., Growth Inhibition and Anti-Cancer Activity of the Human Hepatocarcinoma HepG2 Cell Line in Vitro Study Using Human, Camel and Cow Milk, International Journal of Sciences: Basic and Applied Research , 3, 2, 40-47, (2016).

Park, S. W., Kim, J. Y., Lee, S. J. ve Chung, M. K., A Milk Protein, Casein, as a Proliferation Promoting Factor in Prostate Cancer Cells, The World Journal of Men's Health, 32, 2, 76-82, (2014).

Akca, C., Vatan, O., Yilmaz, D., Huriyet, H., Cinkilic, N. ve Cavas, T., In vitro cytotoxic and genotoxic effects of donkey milk on lung cancer and normal cells lines, Czech Journal of Food Sciences, 37, 29–35, (2019).

Hemmati, A. A., Larki-Harchegani, A., Shabib, S., Jalali, A., Rezaei, A. ve Housmand, G., Wound healing property of milk in full thickness wound model of rabbit, International Journal of Surgery, 54, 133-140, (2018).

Esener, O. B. B., Balkan, B. M., Armutak, E. I., Uvez, A., Yildiz, G., Hafizoglu, M., Yilmazer, N., ve Gurel-Gurevin, E., Donkey milk kefir induces apoptosis and suppresses proliferation of Ehrlich ascites carcinoma by decreasing iNOS in mice, Biotechnic and Histochemistry, 93, 424–431, (2018).

Bumrungpert, A., Pavadhgul, P., Nunthanawanich, P., Sirikanchanarod, A. ve Adulbhan, A., Whey Protein Supplementation Improves Nutritional Status, Glutathione Levels, and Immune Function in Cancer Patients: A Randomized, Double-Blind Controlled Trial, Journal of Medicinal Food, 21, 612–616, (2018).

Tafaro A., Magrone, T., Jirillo, F., Martemucci, G., D’Alessandro, A. G., Amati, L. ve Jirillo, E., Immunological properties of donkey's milk: its potential use in the prevention of atherosclerosis, Current Pharmaceutical Design, 13, 36, 3711-3717, (2007).

Jirillo, F., Jirillo, E. ve Magrone, T., Donkey's and goat's milk consumption and benefits to human health with special reference to the inflammatory status, Current Pharmaceutical Design, 16,7, 859-863, (2010).

Yvon, S., Olier, M., Leveque, M., Jard, G., Tormo, H., Ali, D., Lekhal, H., Peter, M. ve Eutamène, H., Donkey milk consumption exerts anti inflammatory properties by normalizing antimicrobial peptides levels in Paneth’ s cells in a model of ileitis in mice, European Journal of Nutrition, (2016).

Traverso, N., Ricciarelli, R., Nitti, M., Marengo, B., Furfaro, A. L., Pronzato, M. A., Marinari, U. M. ve Domenicotti, C., Role of glutathione in cancer progression and chemoresistance, Oxidative Medicine and Cellular Longevity, (2013).

Badr, G., Camel whey protein enhances diabetic wound healing in a streptozotocin-induced diabetic mouse model: The critical role of β-Defensin-1, -2 and -3, Lipids in Health and Disease, 12, 1, (2013).

Badr, G., Supplementation with undenatured whey protein during diabetes mellitus improves the healing and closure of diabetic wounds through the rescue of functional long-lived wound macrophages, Cell. Cellular Physiology and Biochemistry, 29, 571–582, (2012).

Salam, A., Ebaid, H., Al-Tamimi, J. ve Alhazza, İ., Enhancement of Wound Healing by Un-denatured Camel Whey Proteins in Protein Malnourished Mice, Pakistan Journal of Zoology, 48, 1, 1-9, (2016).

Ebaid, H., Salem, A., Sayed, A. ve Metwalli, A., Whey protein enhances normal inflammatory responses during cutaneous wound healing in diabetic rats, Lipids In Health And Disease, 10, 235 (2011).

Sagliyan, A., Ceribasi, A. O., Gunay, C., Han, M. C., Benzer, F. ve Kandemir, M. F., Effects of dietary supplementation with whey proteins on surgical wound healing in rats, Revue de médecine vétérinaire, 161, 455-462, (2010).

Souroullas, K., Aspri, M. ve Papademas, P., Donkey milk as a supplement in infant formula: Benefits and technological challenges, Food Research International, 109, 416–425, (2018).


Refback'ler

  • Şu halde refbacks yoktur.


Telif Hakkı (c) 2020 Erdal Eroğlu, Cemre Öksüz

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.