Albumin and IgG removal applications with gelatin based biosorbents

Aydan Gülsu, Fatma Ayhan

Öz


Removal of the highest amount of albumin and IgG in the blood plasma for the determination of disease-specific proteins is of great importance in the field of diagnosis and treatment.  In this study it is aimed to prepare the biosorbent to be used as a support material in affinity chromatography. Gelatin biosorbents were prepared by water in oil emulsion method and stabilized with glutaraldehyde as a biofunctional crosslinker to obtain spherical biosorbents. Prepared gelatin biosorbents were characterized by optical micrograph and SEM (scanning electron microcope). It was determined that the size/size distribution of the cellulose biosorbents prepared under  optimal conditions was on average 3-10 µm. The results revealed that 85,7% of albumin and 79% of IgG in the aqua medium was successfully removed with gelatin biosorbents. It is thought that gelatin biosorbents will be very effective in removing albumin and IgG from high concentrations of proteins in the early diagnosis of low concentration proteins.


Anahtar Kelimeler


Gelatin; biosorbent; albumin; IgG; bioaffinity chromatography; proteomic

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Referanslar


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