Neonikotinoid insektisit thiamethoxamın Oreochromis niloticus’ta oksidatif stres parametreleri üzerine etkisi

Özgür Fırat, Tüzin Aytekin

Öz


Araştırmamızda neonikotinoid insektisit olan thiamethoxamın toksik etkileri, Nil tilapyası Oreochromis niloticus’ta oksidatif stres parametreleri ile araştırılmıştır. Bu amaçla balıklar 4 ve 14 günlük sürelerle 60 ve 120 mg/L thiamethoxam etkisine bırakılmış ve solungaç ve bağırsak dokularındaki katalaz (CAT) ve süperoksit dismutaz (SOD) aktiviteleri ile glutatyon (GSH) ve malondialdehit (MDA) düzeyleri belirlenmiştir. Thiamethoxamın etkisinde özellikle de yüksek ortam derişiminde CAT aktivitesinde solungaç ve bağırsak dokularında 4 günlük süre sonunda anlamlı bir artış; 14 günlük süre sonunda ise solungaç dokusunda anlamlı bir azalış belirlenmiştir (P˂0.05). SOD aktivitesi solungaçta her iki etki süresi sonunda insektisidin düşük ve yüksek ortam derişimlerinde; bağırsakta ise 14 günlük süre sonunda ve yüksek ortam derişiminde artış göstermiştir (P˂0.05). GSH düzeyi özellikle de 120 mg/L thiametoxam etkisinde her iki dokuda da 4 günlük süre sonunda anlamlı bir artış; 14 günlük süre sonunda ise önemli bir azalış göstermiştir (P˂0.05). Solungaç ve bağırsak dokularındaki MDA düzeyi ise, 14 günlük süre sonunda thiametoxamın yüksek ortam derişiminde önemli düzeyde artmıştır (P˂0.05). Sonuç olarak çalışmamız, thiametoxamın özellikle de yüksek ortam derşiminde O. niloticus’ta oksidatif strese neden olduğunu göstermektedir.

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