Gıdalarda koruyucu katkı maddesi olarak kullanılan sodyum benzoat ve potasyum sorbat karışımının genotoksik potansiyelinin mikronukleus testi ile belirlenmesi

Sevcan Mamur, Nazmiye Ataseven, Fatma Ünal, Deniz Yüzbaşıoğlu

Öz


Sodyum benzoat (SB) ve potasyum sorbat (PS), gıdalarda koruyucu katkı maddesi olarak yaygın bir şekilde kullanılmaktadır.  Bu çalışmada SB ve PS karışımının genotoksik potansiyeli insan lenfositlerinde mikronukleus (MN) testi ile değerlendirilmiştir.  SB+PS karışımının dokuz farklı kombinasyonu (12.5 SB+125 PS ile 50 SB+500 PS μg/mL aralığında) kültüre alınmış insan lenfositlerine 48 saat uygulanmıştır.  MN testi sonuçlarına göre; SB+PS karışımının uygulanan tüm konsantrasyonlarda mikronukleus frekansını kontrole kıyasla anlamlı düzeyde artırdığı belirlenmiştir.  Buna karşın, SB+PS karışımının çalışılan konsantrasyonlarda hücre siklusu belirteci olan nükleeer bölünme indeksini (NBİ) anlamlı düzeyde etkilemediği tespit edilmiştir.  Elde edilen sonuçlar, SB+PS karışımının in vitro koşullarda MN testi ile insan lenfositlerinde klastojenik ve/veya anöjenik etkileri olabileceğini göstermektedir.

 


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