Role of pH on the initiation of sperm motility in rainbow trout (Oncorhynchus mykiss) and Çoruh trout (Salmo coruhensis)

Filiz Kutluyer

Öz


Experiments were designed to examine effects of pH (range 6.0–9.0) in different activation solutions on sperm motility of rainbow trout (Oncorhynchus mykiss) and Çoruh trout (Salmo coruhensis). Four activation solutions were prepared: a) NaCl (52 mM)-AS1, b) NaCl (103 mM), CaCl2 (63 mM), Glucose (4 mM), Tris (10 mM)-AS2, c) NaHCO3 (119 mM)-AS3, d) NaCl (45 mM), KCl (5 mM), Tris (30 mM)-AS4. Sperm was activated at the dilution ratio of 1:100 with different activation solutions. Highest motility rate and duration for O. mykiss were obtained from AS2 at a pH of 9 while highest motility rate and duration for S. coruhensis were at a pH of 8. Data here reported also showed that non-progressive motility in a range of 6–8 was observed in AS2 for two species. In conclusion, rainbow trout (O. mykiss) and Çoruh trout (S. coruhensis) sperm cells can be motile in a wide range of pH although sperm motility and duration was negatively influenced by low pH values.


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