Investigation effects of some anthraquinones on human paraoxonase 1 (hPON1)

Başak Gökçe

Öz


The in vitro effects of the some dihydroxyanthraquinone derivates, anthrorufin, xanthopurpurin, chrysazin and chrysaphanol which are commonly used as anticancer antibiotics, on the activity of purified  serum paraoxonase 1 (PON1) (EC 3.1.8.1.) were investigated.  Paraoxonase 1 was purified from human blood serum 355,12 fold with 55 % yield with use of ammonium sulphate precipitation and Sepharose-4B-L-tyrozine-1-napthylamine HIC (hydrophobic interaction chromatography) coloumn in return.  Sodium Dodecyl Sulphate -polyacrylamide gel electrophoresis (SDS-PAGE) analysis of purified enzyme showed a single band belong to human serum PON1, 43 kDa.  The four anthraquinones inhibited PON activity strongly, with IC50 values for anthrorufin, xanthopurpurin, chrysazin and chrysaphanol of 73, 72.25, 75.19 and 105.53 M respectively.  Ki constants were determined 34.64, 39.51, 39.73 and 63.59 M, respectively.  All anthraquinones showed inhibition in competitive manner.


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